Some reasons why a breeder may choose artificial insemination over natural breeding.
Dogs that unable to breed in a natural way due to injury. Their condition may be permanent or temporary but not an injury that is a genetic imperfection.
Size..Sometimes dogs and bitches are not always size compatible. Just because a male is smaller than the female is no reason not to breed them. The male may not be able to complete the breeding. Then Artificial insemination will be the right path to try.
Bitches that have developed crushes on specific stud and may not let a stud that you have chosen for breeding near her. She may aggressively inclined toward the stud and try to take his face off if he trys to mount her.
Sometimes a stud may be lukewarm on the breeding process when presented with elderly bitches or bitches who have had known repro problems in the past. Chances that your stud has a valid reason is a good one and only he knows the reason why not to breed. Your stud has aa amazing nose. They are not born with it and requires education and experence to develop it's nose. A good stud will not waste his time mounting and trying to breed a bitch that does not have eggs ready for fertilization. He may not want to waste a breeding on eggs that fertilized a couple days ago. His nose will tell him if eggs are there and if they are ready for fertilize. Your less experienced stud may be a little less reliable. Sometimes your older studs will let him know several times that the bitches are his and that his advances to them are unwelcome. This may keep your young stud not to develope the nose.
Sometimes the stud and bitch are in two places and require shipment of sperm for artificial insemination.
Make sure the dog has recently urinated before the collection begins. Techniques vary on the size of the dog. A 4.5lbs toy size dog and 150lbs mastiff. The general overall picture of collecting is the same. Small dogs and some medium dogs can be collected on the counter top or grooming table. Larger dogs you will have to sit on the floor with a small stool and may need two people for the collection. Your most successful collections will be those using a teaser bitch. Most of the time the teaser bitch is the one that will be inseminated but not always.
Teaser Bitch If your stud has already rejected the bitch that is to be inseminated, she most likely will not make a suitable teaser bitch. If you have another female in season you can use her and then inseminate the intended mom to be. If you don't have another female in seasons you still have other options. If you have a female that has a litter and nursing she may do well as a teaser bitch. You must be quick before he catches on that she only smells different because she is nursing and not in season. Another helpful tool is to use a cotton-tipped applicator and swab them lightly around inside the vulvas of the girls who are in full-blown season and then freeze them inside a sandwich baggy. Then all you have to do is thaw them for a few minutes at room temperature and swab the rear end of the teaser bitch.
The Collection Process
You can get your supplies on the internet. For the collection bag you can use a Playtex baby bottle inserts. Use the ones that is labeled “Drop Ins”. We get our A.I. Supplies from
Allow the dog to do some sniffing. If he does not mount the bitch, stroke his prepuce a few times until you feel that he is getting an erection. You may have to lift him in the mounting position to encourage him. Most of the time you will not have to do anything to get him to mount the bitch.
Once he mounts the bitch quickly place the collection bag over his penis and push the prepuce up and behind the bulb. The prepuce must be behind the bulb if not the dog can not become fully erect and the process will be very painful for him. Do not allow him to become erect inside of the prepuce.
You will be letting your hand mimic the “feel” of the vagina by holding his penis so that you can put slight pressure behind the bulb of the penis. It will help to make sure your hands are warm.
Once the dog begins the thrusting actions you are pretty assured of getting a collection. Continue holding the penis until you have collected the entire sperm fractions and some of the prostate fluid.
The prostate fluid is clear like water. The sperm fraction is very thick and milky white. When the two are mixed the semen will be cloudy but thinned out and watery. You must be gentle with it and not cause any bubbles because they will be very difficult to get out of your insemination rod.
Gently remove the collection bag from the penis and put the dog away in a safe place. Check to make sure his penis has retracted back into the prepuce.
Studies have shown that you will maximize the quantity of collection when using a teaser bitch.
Attach the syringe to the insemination rod. Pull the semen into the syringe by pulling the end of the rod into the semen in the collection bag. Holding the syringe and rod upward and with a light push out all the air from the syringe and rod pushing semen almost to the tip of the rod.
You may want to check to make sure the semen is alive and active by placing a single drop of semen on a slide and using a microscope.
Now you are ready for the Insemination process.
Make sure that the bitch has emptied her bladder prior to the insemination. With larger bitches you may need to have a second person to help. The best tool to use is an insemination rod. Some breeders use soft flexible catheters but you may have better luck with the rods and sometimes the soft flexible catheters may contain latex which will kill the sperm. Make sure your syringes are labeled “latex free”. After you have completed all the A.I.s , throw away the rod and syringe away. Do not use them on another bitch. You can clean between A.I.s by rinsing well in very hot water, followed by a rinse in normal saline solution and let them completely dry in a sunny window before using for a 2nd and 3rd time on the same bitch.
Time to make some pups
Time to make some puppies…
At this point you should have a syringe and insemination rod with no air bubbles. With someone hold the front end of the bitch. You may not need anyone to hold the front end if your bitch is small. Insert the rod going up into the vulva aiming towards the spine. The rod should be almost parallel to the legs. Do not insert the rod going straight into the vulva. The vulva and vagina are shaped like an upside down L. Insert until you feel it comes to the dead end, which would be up next to the spine. Then lift the rod upwards until the rod is now pointed straight into the vulva and gently move the rod forward. This is why a flexible rod is not desirable. You need the firmness of the rod to “lift” the vulva so the rod can then go straight in towards the cervix. Advance the rod into the vagina until you can not advance it any further. When you have done this a few times you will learn the correct depth for your bitch.
When you have the rod in place hold the bitch with her back legs up in the air push in the plunger of the syringe placing all the semen . Once the semen has been placed disconnect the syringe and pull one cc of air and reconnect to the syringe and push the air into the bitch. This is to clear all the semen out of the rod.
Lightly massage the vulva area. You may feel this area “clasping”. This is an indication that the bitch’s body is pulling the semen up into her uterus. Keep her in a crate for a few hours. You will repeat just like if you were using a stud, the next day or the second day after.